Small polydisperse circular dna of hela cells
WebApr 12, 2024 · Of note, the cells were exposed to different stresses after 24 h of initial cell seeding, challenging HeLa cells as follows: Staurosporine and Rapamycin were administrated at a final concentration of 10 µM and 20 nM, respectively, whereas H 2 O 2 was administrated to reach 50 µM in the cell solution. For the hypotonic stress, the cells … WebExtrachromosomal circular DNA (eccDNA) is a closed-circle, nuclear, nonplasmid DNA molecule found in all tested eukaryotes. eccDNA plays important roles in cancer …
Small polydisperse circular dna of hela cells
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WebJan 1, 2001 · Previous studies have suggested that eccDNA is generated through a process involving recombination between repetitive sequences. However, we observed that … WebIntroduction. Extrachromosomal circular DNA (eccDNA) is a type of cell free DNA (cfDNA) which is more structurally stable than linear cfDNA currently used for cancer-related detections under clinical settings. 1,2 Although eccDNA was first discovered in 1965 by Hotta and Bassel et al in boar sperm using an electron microscope, further research on …
WebSep 3, 2024 · EccDNAs with a length less than 500 bp are called small polydisperse circular DNA (spcDNA) [17, 29–32], and a few spcDNA molecules are hybridized to unique sequences [29, 31–33]. The eccDNA distribution is not restricted to … http://archive.nstl.gov.cn/Archives/browse.do?action=viewDetail&articleID=f0891a987daedbab&navig=9565bcbb40dbfbe9&navigator=category&flag=byWord&subjectCode=null&searchfrom=null
WebMar 8, 2012 · The circular DNA from mouse tissues and cell lines were 80 to 2000 bp long, ... et al.., Purification of small polydisperse circular DNA of eukaryotic cells by use of ATP-dependent deoxyribonuclease. ... Murnane J. P., Formation of extrachromosomal circular DNA in HeLa cells by nonhomologous recombination. Nucleic Acids Res. 22, 2447 (1994 ... WebEukaryotic cells often contain a population of small polydisperse circular (spc) DNAs with molecular weights ranging from 1 X 105 to 4 X 106. ... sophila (4), and BSC-1 (3) cells. Specifically, HeLa spcDNA appears to be predominantly cytoplasmic, present at a level of ... small circular DNA from these tissues, 5-10 g of bursa or 30-40 g of ...
WebHuman Genetics 91 , 489–495 ( 1993) Cite this article 56 Accesses 16 Citations Metrics By screening total human DNA with probes derived from the small polydisperse circular (spc) DNA fraction of cultured human cells, we identified three clones that carry long stretches of β-satellite DNA.
the queens leeds hotelWebof populations of DNA molecules of essentially identical sequence. The most thoroughly characterized spcDNA is that from three established cell lines: Drosophila Schneider's line 2 cells (8,9), AGM BSC-1 cells (11,12,13), and HeLa cells (10). The small circular DNAs from Schneider's cells have a mean length of 3.3kh and range in size from ... sign in report azureWebThe entire DNA sequence of one clone was determined and found to he alphoid satellite with a variant monomeric construction. INTRODUCTION Small polyeisperse circular (spc) DNA … sign in representativeWebSmall polydisperse circular DNA of HeLa cells (Q70476021) From Wikidata. Jump to navigation Jump to search. scientific article published on 01 August 1972. edit. Language … sign in required we can\\u0027t upload or downloadWebJul 11, 1994 · Previous studies have suggested that eccDNA is generated through a process involving recombination between repetitive sequences. However, we observed that approximately one half of the small eccDNA fragments cloned from HeLa S3 cells were composed entirely of nonrepetitive or low-copy DNA sequences. sign in required什么意思WebThe size of small polydisperse circular DNA (spcDNA) in angiofibroma-derived cell cultures from patients with tuberous sclerosis (TSC) differs from that in fibroblasts. Human … sign in · rightfax mountsinai.orgWebOct 25, 1989 · An abundant class of HeLa extrachromosomal circular DNA containing the transposon-like element, THE-1, is shown to arise via site specific recombination. The chromosomal locus from which these circles are derived, however, is single-copy. Northern blot analysis detects homology to two polyadenylated RNAs in HeLa cells. sign in required windows 10