P2a-tdtomato
http://www.taitool.com/pr.jsp?_jcp=3_97 WebJan 1, 2024 · The two-photon laser was tuned to the excitation wavelength for tdTomato (1000 nm). All experiments were performed using a 1.1-numerical aperture (NA) 25× objective lens immersed in ACSF to obtain 62.5X high-magnification (166.4 μm × 166.4 μm; 512 × 512 pixels; 1.5-μm step) images for neuronal analysis.
P2a-tdtomato
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WebTdTomato reporter induction efficiency was determined by analyzing retinal whole mounts utilizing confocal microscopy, using the antibodies Anti-neural/glial antigen 2 (PCs), Anti … WebNational Center for Biotechnology Information
WebJan 8, 2024 · Scrt2-P2A-tdTomato/+ It is a knockin mouse line where tdTomato can reflect Scrt2 mRNA expression pattern: Jackson Lab: stock #: 034390 (will be deposited soon) Strain, strain background (Mus musculus) Celf4-3xHA-P2A-iCreER-T2A-EGFP/+ It is a knockin mouse line where HA is tagged at Celf4 protein c terminus. WebC57BL/6-Ly6g em2(iCre-P2A-tdTomato-polyA)Smoc: exact match to ID Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse …
WebTamoxifen诱导Cre-ERT2小鼠 使用指南. Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。 WebNov 29, 2024 · Here, we have demonstrated that transient selection with puromycin or zeocin can facilitate highly efficient scarless KI of relatively large reporter genes (over 2 kb in the case of BRN3B-P2A-tdTomato-P2A-Thy1.2) into both human and mouse PSCs. These KI cell lines differentiated normally, and did not show karyotypic abnormalities or display …
WebJul 27, 2024 · We treated PDGFRβ-P2A-CreER T2; Rosa-tdTomato moms with a single dose of tamoxifen on the day of birth (P0) and analyzed the retinal vasculature of the …
WebAug 4, 2024 · Generating the Atoh1- 3*V5-P2A-Tdtomato/ + Mouse Strain for Neonatal Cochlear HC Sorting and ATAC-Seq Analysis. To search for previously unreported Atoh1 enhancers, we used neonatal (P1) cochlear HCs to perform ATAC-seq, a widely used approach to identify open chromatin regions where enhancers are likely located ( 33, 39 ). is german an indo european languageWebDec 21, 2024 · The GlyT2-P2A-iCre and GlyT2-tdTomato mice were successfully constructed. GlyT2-tdTomato fluorescence was colocalized extensively with immunoreactivity of glycine, GlyT2 and Pax2 in somata, confirming the selective expression of the transgene in glycinergic neurons. GlyT2-tdTomato neurons were mainly … sa chat sitesWebApr 10, 2024 · The same AAV was also injected into LSL-tdTomato homo mice to test SB100-P2A-Cre fusion protein expression. (C) Liver sections from the validation mice described in (B). H&E and PTEN IHC staining showed that the frequency of PTEN deficient hepatocytes correlated with the amount of AAV injected. sa children\u0027s hospitalWebA next-generation trading platform driven by machine learning and AI that allows anyone with an interest in crypto to easily automate their portfolio across wallets, exchanges, and … sa checklist icaiWebPlasmid pCDH-CB-FLPe-P2A-tdTomato from Dr. Kazuhiro Oka's lab contains the insert FLPe-P2A-tdTomato and is published in Unpublished This plasmid is available through … sa cha shrimp recipeWebDec 18, 2024 · In zebrafish, mutation of tryptophan hydroxylase 2 ( tph2 ), which is required for serotonin (5-HT) synthesis in the RN, results in reduced sleep, sleep depth, and homeostatic response to sleep deprivation ( Oikonomou et al., 2024 ). Pharmacological inhibition of 5-HT synthesis or ablation of the RN also results in reduced sleep. sa check in app link medicareWebJun 27, 2016 · Here we report the production of a BRAF-P2A-tdTomato ( BrafTOM) mouse model. We show that the BrafTOM mouse expresses bright tdTomato in several organs and cell types known to rely on BRAF signaling for normal functions, including the brain, hair follicles, and lungs. sa child benefit