Eyeless gal4
Tīmeklis2008. gada 21. okt. · We also analyzed adult weight and wing size and larval hemolymph glucose/trehalose concentrations in animals expressing a dominant-negative form of Eyeless with the eyeless Gal4 driver OK107 (25, 26) or with the dilp2 Gal4 driver . These experiments gave results similar to those obtained with mutant … Tīmeklis2014. gada 23. maijs · We expressed the RNAi lines using the eye-specific eyeless-Gal4 and GMR-Gal4 and observed small eye phenotypes from both skd and kto knockdown flies, similar to the documented loss-of ... Newly eclosed flies with Mef2-Gal4–driven knockdown of skd or kto were fed normal food for 12 d and then moved …
Eyeless gal4
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Tīmeklis2024. gada 15. febr. · ey-Gal4, eyeless-GAL4, eyGAL4, EYE-GAL4, ey>Gal4, ey-G4, eyelessGal4, eyeless GAL4, P{ey-Gal4}, ey. Size Expression Data GAL4 … TīmeklisAbstract. Over the past decade the adoption and refinement of the GAL4 system by the Drosophila field has resulted in a wide array of tools with which the researcher can …
http://hawaiireedlab.com/wpress/?p=477 Tīmeklis2024. gada 2. dec. · By placing the eyeless gene under the regulation of GAL4, the gene can be activated wherever gene activator GAL4 is expressed. When these two …
Tīmeklis1995. gada 24. marts · The Drosophila gene eyeless (ey) encodes a transcription factor with both a paired domain and a homeodomain. It is homologous to the mouse Small … Tīmeklis1999. gada 1. aug. · Second and third EGUF (Eyeless-GAL4 UAS-FLP) chromosomes containing both ey-GAL4 and UAS-FLP were generated by meiotic recombination. The original pGMR-hid insertion was obtained from Hermann Steller (described in Grether et al. 1995). This second chromosome GMR-hid insertion was recombined onto FRT …
Tīmeklis1998. gada 19. nov. · All combinations of eyeless–Gal4 and different UAS lines were created at 25 °C. Generation of mosaics. Clones of the fng 13 allele 4 were induced in first/second-instar larvae of yw hsp-70-flp; ...
TīmeklisThe GAL4/UAS system is commonly used in Drosophila to drive expression of a gene of interest ( Brand & Perrimon, 1993). Tissue-specific or ubiquitous enhancers drive expression of GAL4 that results in transcriptional activation from the GAL4 binding sites (UAS) to drive expression of a gene of interest, and is widely used for expression of ... picnic offensive university of michiganTīmeklisWe used eyeless-GAL4 from Domínguez and de Celis (1998) as the main GAL4-driver, but confirmed the primary phenotypes with the similar line ey3.5-GAL4 (BL-8220) and the unrelated driver so-GAL4 ... picnic of the worldTīmeklis2013. gada 16. maijs · The GAL4/UAS binary expression control system has been an extremely useful tool in Drosophila genetics. ... Now … picnic of the world lyricsTīmeklis2024. gada 26. maijs · eyeless-Gal4-driven RNAi against each of these genes resulted in high frequencies of flies with reduced eye sizes. RNAi against dTgs1 produced small eye discs containing a reduced proportion of Elav-positive differentiated cells compared to … top bank california escondidoTīmeklis2013. gada 28. marts · The EGUF system carrying the eyeless-Gal4 driver was used to drive the UAS-RNAi transgenes in the eye and also makes the eye homozygous for MAGE (sst RZ). Controls for the effects of each eyeless-driven RNAi alone were carried out for ATM and ATR resulting in wild type appearing eyes, but only the results of … picnic olympic gamesTīmeklis2024. gada 19. okt. · We obtained an RNAi line for the rrp4 gene from the VDRC stock center and crossed it to the eyeless-GAL4 (ey-Gal4) line expressed in the optic lobes at early developmental stages, as well as the ubiquitously expressed daughterless-GAL4 (da-Gal4) driver. ey-Gal4-driven rrp4 knockdown resulted in small and rough eyes , … top bank checking accountsTīmeklisAct5C-GAL4 (ubiquitous; Ito et al., 1997) lethal lethal no phenotype apterous-GAL4 (dorsal wing/notum; Calleja et al., 1996) lethal lethal no phenotype eyeless-GAL4 (eye, head capsule, CNS; Hazelett et al., 1998) lethal lethal no phenotype MS1096-GAL4 (wing pouch; Capdevila and Guerrero, 1994) wings reduced in size with upward … picnic ohne app